The proposed research will demonstrate the effectiveness of specific genes in canola for resistance to sclerotinia. Plant breeders will be able to select QTLs to increase the likelihood of capturing these resistance genes in breeding lines.

The current project aims to define a root system architecture RSA that contributes to improved NUE for canola and will allow the reduction of nitrogen inputs while maintaining productivity. With increasing temperatures predicted for the Prairies in coming years it is becomes imperative to generate climate resilient crops.

The differential lines will provide canola pathologists and breeders with an extremely valuable tool for assessing the effectiveness of resistance. They will be made available to the canola industry for variety development, which will ensure that Saskatchewan producers have a diverse range of clubroot resistant cultivars to select from.

Genetic resistance is considered as the most efficient method for control of blackleg. Previous research results indicate that both Canadian and Chinese B. napus varieties could carry novel genes for resistance to blackleg. Therefore, it is necessary to identify and map the unknown R genes in the canola varieties.

There is a pressing need to improve productivity of crops, in order to maximize yield without further expanding arable land. The ability to make further crop improvements relies on the introduction of novel allelic variation, one such source being related species; however, interspecific barriers to recombination limit the transfer of new variation into crops.

The tools developed and verified through this project will enable efficient development of resilient varieties. The results support potential of canola digital phenotypes to field-scale agronomic applications. The expansive data sets and samples generated through this project are and will be used in various research projects, extending the utility of grower-invested research dollars.

Seed of PAK93-based pre-breeding lines will be more attractive to plant breeding companies than the original germplasm such as PAK54, primarily because it will be faster to develop hybrid varieties that combine the desirable traits from PAK93-derived lines with other important traits such as herbicide tolerance and resistance to the diseases, blackleg and clubroot.

Increase canola protein inclusion rates in monogastric animal feeds, followed by canola germplasm that produces protein better suited for human diets, and finally specialty varieties that produce protein for specific technical applications.

The proposed research will deliver knowledge and tools to improve utilization of existing clubroot resistant cultivars and to accelerate the discovery of new clubroot resistance genes, with the anticipation of exploring broad-spectrum and durable clubroot resistance that will be highly beneficial to breeders and growers of canola and other Brassica crops.

The pathogen (P. brassicae) populations in western Canada is evolving rapidly. It is extremely important to have canola cultivars with new sources of resistance avialabe to canola producers in Saskatchewan.

The Western College of Veterinary Medicine (WCVM) was the first veterinary college in North America to begin a honey bee research and teaching program, recognizing the high importance of pollinators for the long-term sustainability of agriculture and food security.

This facility will increase the overall capacity for insect and pest related research and will therefore benefit numerous researchers and projects at the USask. Finally, this facility will result in important training opportunities.

Pathotying with the CCD or race profiling with the NILs is a phenotyping based approach, which can be tedious and time consuming. A genotyping based method could be an ultimate solution for race profiling.

This is the first investigation into the infection by pycnidio- and asco-spores, as well as a mixture via wounds under the influence of cultivar resistance and fungicide seed treatment for blackleg disease in canola. The findings from this study suggested that it is feasible to produce ascospores and pycnidiospores in the lab and use these types of spores to test canola materials for blackleg resistance in both controlled environment and field conditions.

Clubroot poses a significant threat to canola growers. Although genetic resistance is critical for managing the disease, changes in pathogen virulence endanger its effectiveness. Second-generation resistance offers promise against pathotypes that can overcome first-generation resistance. However, without an understanding of pathogen virulence on second-generation resistance, we risk compromising its efficacy. This project provided data on the performance of second-generation clubroot-resistant cultivars in the field, along with insights into the virulence characteristics of pathogen populations collected from these cultivars.

Understanding weed biology is the first BMP for reducing risk of herbicide resistance. This study will result in an understanding of different cleavers biotypes that exist across the Prairies, their emergence phenology, and some of their biological characteristics.

This work will provide crucial information needed by the agricultural industry and growers: field-scale data on how farmers may best balance agronomic and environmental outcomes by adjusting either the timing of fertilizer application or the fertilizer formulation.

Researchers and breeders will have greater confidence that the clubroot material they are working with is actually the correct pathotype, and that a pathotype shift hasn’t occurred part way through their research experiment and/or clubroot resistance breeding program.

The study will compare 1) uncoated and 2) polymer coated mono-ammonium phosphate, 3) liquid ammonium polyphosphate, 4) diammonium phosphate, 5) triple superphosphate, 6) ammonium phosphate sulfate, 7) struvite and 8) granular rock phosphate in broadcast versus banded placement.

This will accelerate the breeding of improved canola varieties as breeders from academia/government/industry use this service to phenotype larger breeding programs. This builds research capacity at GIFS, and will provide a useful phenotyping platform that will be utilized in future research proposals.