Purifying genotypes of Plasmodiophora brassicae and developing SNP markers linked to races of P. brassicae populations collected in western Canada

Term: 5 years, beginning in 2021
Status: Ongoing
Researcher(s): Fengqun Yu, AAFC
SaskCanola Investment: $224,813
Total Project Cost: $599,501
Funding Partners: Western Grains Research Foundation, Manitoba Canola Growers Association

Project Description

Pathotying with the CCD or race profiling with the NILs is a phenotyping based approach, which can be tedious and time consuming. A genotyping based method could be an ultimate solution for race profiling. A method for rapid genotyping of P. brassicae pathotypes in field populations will allow producers to choose canola varieties that are resistant to the predominant pathotypes found in their field, and also will allow stewardship for the longest durability of resistance genes. Clubroot resistance breeding will be accelerated, and clubroot resistance labelling will become possible (similar to blackleg resistance labelling) based on genotyping.

Objectives

1. Develop an efficient method to produce near pure genetic isolates (NPGI) of P. brassicae

2. Produce diverse NPGIs from clubroot galls collected in western Canada

3. Determine race profile for each NPGI

4. Carry out genome sequencing of selected NPGIs

5. Develop SNP markers tightly linked to each Avr gene and obtain pure genotype isolates of P. brassicae