Agronomy SaskCanola Agronomy SaskCanola

A simple and holistic approach to alleviate biotic and abiotic stresses in canola through silicon (Si) uptake

This project intends to fully characterize the properties of canola with respect to its instrinsic ability to absorb Si, means to improve its ability, and the benefits that canola could derive from this in terms of disease protections with special emphasis on the most important ones: blackleg, clubroot, and sclerotinia.

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Pre-breeding Tools SaskCanola Pre-breeding Tools SaskCanola

Towards better understanding of genetics in Leptosphaeria-Brassica interactions via international collaborations to standardize the nomenclature of blackleg resistance genes

The best approach to manage blackleg disease is the use of canola cultivars that are genetically resistant to the pathogen. However, cultivars that contain the resistant (R) gene(s) against the most prevalent pathogen race(s) are more likely to be effective in controlling blackleg disease. Among the various tools developed from this and other similar projects, markers for race determination of blackleg pathogen and markers that determine the type of R gene in canola cultivars have the most practical and immediate benefit for canola farmers by helping them to achieve both goals.

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Agronomy SaskCanola Agronomy SaskCanola

From field to the genome. Application of 3rd generation sequencing to direct genotyping of canola pathogens

We applied target capture sequencing to canola root galls and soil samples from three fields in Alberta. Sequencing data showed that the clubroot pathogen pathotype 3H was present in two fields. A third field sample showed presence of new mutations in one of the target sequences indicating presence of clubroot pathotype 3H and potentially other pathotype that were not present in our clubroot sequence dataset. We also determined the genotype of blackleg races from three canola stems infected with blackleg and determined a mixture of blackleg species as well as other pathogenic fungi present in these samples.

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Pre-breeding Tools SaskCanola Pre-breeding Tools SaskCanola

Increasing abiotic (drought) and biotic (clubroot) resistance in Brassica species (Arabidopsis and Canola) by modifying auxin response

This project focused on increasing our knowledge on plant host-clubroot pathogen interactions by determining if reducing the ability of the pathogen to use the plant hormone auxin (responsible for cell grow, division and expansion in the plant) would reduce clubroot disease progression, particularly at the gall forming stage.

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Pre-breeding Tools SaskCanola Pre-breeding Tools SaskCanola

Defining populations of Plasmodiophora brassicae with near isogenic Brassica napus lines

The differential lines will provide canola pathologists and breeders with an extremely valuable tool for assessing the effectiveness of resistance. They will be made available to the canola industry for variety development, which will ensure that Saskatchewan producers have a diverse range of clubroot resistant cultivars to select from.

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Pre-breeding Tools SaskCanola Pre-breeding Tools SaskCanola

Identification and genetic mapping of novel genes for resistance to blackleg in Chinese and Canadian Brassica napus varieties

Genetic resistance is considered as the most efficient method for control of blackleg. Previous research results indicate that both Canadian and Chinese B. napus varieties could carry novel genes for resistance to blackleg. Therefore, it is necessary to identify and map the unknown R genes in the canola varieties.

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Pre-breeding Tools SaskCanola Pre-breeding Tools SaskCanola

Manipulating recombination in crop polyploids

There is a pressing need to improve productivity of crops, in order to maximize yield without further expanding arable land. The ability to make further crop improvements relies on the introduction of novel allelic variation, one such source being related species; however, interspecific barriers to recombination limit the transfer of new variation into crops.

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Pre-breeding Tools SaskCanola Pre-breeding Tools SaskCanola

Addressing yield stability drivers of canola in a changing climate using high throughput phenotyping

The tools developed and verified through this project will enable efficient development of resilient varieties. The results support potential of canola digital phenotypes to field-scale agronomic applications. The expansive data sets and samples generated through this project are and will be used in various research projects, extending the utility of grower-invested research dollars.

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