Identifying the optimal root system architecture (RSA) for Brassica crops
The current project aims to define a root system architecture RSA that contributes to improved NUE for canola and will allow the reduction of nitrogen inputs while maintaining productivity. With increasing temperatures predicted for the Prairies in coming years it is becomes imperative to generate climate resilient crops.
Defining populations of Plasmodiophora brassicae with near isogenic Brassica napus lines
The differential lines will provide canola pathologists and breeders with an extremely valuable tool for assessing the effectiveness of resistance. They will be made available to the canola industry for variety development, which will ensure that Saskatchewan producers have a diverse range of clubroot resistant cultivars to select from.
Identification and genetic mapping of novel genes for resistance to blackleg in Chinese and Canadian Brassica napus varieties
Genetic resistance is considered as the most efficient method for control of blackleg. Previous research results indicate that both Canadian and Chinese B. napus varieties could carry novel genes for resistance to blackleg. Therefore, it is necessary to identify and map the unknown R genes in the canola varieties.
Manipulating recombination in crop polyploids
There is a pressing need to improve productivity of crops, in order to maximize yield without further expanding arable land. The ability to make further crop improvements relies on the introduction of novel allelic variation, one such source being related species; however, interspecific barriers to recombination limit the transfer of new variation into crops.
Addressing yield stability drivers of canola in a changing climate using high throughput phenotyping
The tools developed and verified through this project will enable efficient development of resilient varieties. The results support potential of canola digital phenotypes to field-scale agronomic applications. The expansive data sets and samples generated through this project are and will be used in various research projects, extending the utility of grower-invested research dollars.
Pre-breeding lines combining canola quality with sclerotinia resistance, good agronomy and genomic diversity from PAK93
Seed of PAK93-based pre-breeding lines will be more attractive to plant breeding companies than the original germplasm such as PAK54, primarily because it will be faster to develop hybrid varieties that combine the desirable traits from PAK93-derived lines with other important traits such as herbicide tolerance and resistance to the diseases, blackleg and clubroot.
Enabling Canola Protein Optimization (ECPO)
Increase canola protein inclusion rates in monogastric animal feeds, followed by canola germplasm that produces protein better suited for human diets, and finally specialty varieties that produce protein for specific technical applications.
A proteomics-based approach towards identifying host and pathogen proteins critical to clubroot establishment in canola
The proposed research will deliver knowledge and tools to improve utilization of existing clubroot resistant cultivars and to accelerate the discovery of new clubroot resistance genes, with the anticipation of exploring broad-spectrum and durable clubroot resistance that will be highly beneficial to breeders and growers of canola and other Brassica crops.
Cloning clubroot resistance genes from B. nigra and transferring the genes into canola through a CRISPR/Cas9 based technology
The pathogen (P. brassicae) populations in western Canada is evolving rapidly. It is extremely important to have canola cultivars with new sources of resistance avialabe to canola producers in Saskatchewan.
Developing allele specific molecular markers for the B.napus blackleg resistance (Rlm) genes
Our knowledge of pathogen virulence genes and plant race specific resistance (R) genes in the Leptosphaeria-Brassica pathosystem has tremendously advanced in the past two decades.
Functional validation of Brassica napus genes related to clubroot resistance through high-throughput CRISPR/Cas9 genome editing
Growing clubroot-resistant (CR) cultivars in appropriate rotations remains the most effective solution to the long-term management of clubroot disease.
Understanding the molecular basis of NLR-mediated clubroot resistance in Brassica napus
The ability to integrate stable clubroot resistance into new germplasm is needed to protect the economy while ensuring sustainability and growth in canola growing regions.
Modification of surface waxes for improved water retention in canola
Crop residues supply critically needed carbon (C) and nutrients to the soil. These residue-derived resources support plant growth and the formation of soil organic matter, a cornerstone of soil health.
Deploying calcium-dependent protein kinases to fight canola pathogens
This project aims to contribute to the development of elite canola varieties that are resistant to pathogen infection for the betterment of the canola industry.
Generation of canola lines with increased heat and drought tolerance by regulating phospholipid: diacylglycerol acyltransferase activity
This project will help improve canola production efficiency and improve the national and global competitiveness of the Canadian canola industry (in the long term).
Drought tolerance in Canola through modulating the Kanghan (KH) gene family
Improving crop tolerance to drought is essential for maintaining yield stability under the continued threat of climate change and a key factor for achieving sustainability in agriculture by saving water resource usage. Molecular breeding focusing on monogenic transgenic intervention has so far achieved limited success in the development of drought tolerance in crops. This project focuses on a gene family that is the basis of quantitative trait loci (QTL) affecting drought tolerance in Brassicaceae.
Identification and exploitation of genome structural variants for trait improvement in Prairie crops
This has the potential to open up plant breeding to a whole new way to identify germplasm variation in breeding programs.
Genome wide functional analysis of Plasmodiophora brassicae effectors and the management of clubroot disease
Clubroot disease in canola and other brassicas is caused by the pathogen Plasmodiophora brassicae. Researchers at the University of Saskatchewan conducted a five-year study to identify and characterize effector proteins and their role in the establishment and progression of clubroot disease.
Yu: Introgression of Disease Resistance from Brassica nigra into Canola using a new-type B. napus
New sources of clubroot and blackleg resistance are needed in western Canada because virulent pathogen populations have been reported that are able to overcome the resistance of canola cultivars for both diseases.
Peng: Developing Canola Germplasms with Diverse Mechanisms to Enhance the Durability of Clubroot Resistance
Cultivar resistance is considered the most effective and practical approach for clubroot management. However, current resistant canola cultivars, available in Canada since 2009, were based on a single clubroot resistance (CR) gene.