Agronomy SaskCanola Agronomy SaskCanola

From field to the genome. Application of 3rd generation sequencing to direct genotyping of canola pathogens

We applied target capture sequencing to canola root galls and soil samples from three fields in Alberta. Sequencing data showed that the clubroot pathogen pathotype 3H was present in two fields. A third field sample showed presence of new mutations in one of the target sequences indicating presence of clubroot pathotype 3H and potentially other pathotype that were not present in our clubroot sequence dataset. We also determined the genotype of blackleg races from three canola stems infected with blackleg and determined a mixture of blackleg species as well as other pathogenic fungi present in these samples.

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Agronomy SaskCanola Agronomy SaskCanola

WCVM Research Chair in Pollinator Health

The Western College of Veterinary Medicine (WCVM) was the first veterinary college in North America to begin a honey bee research and teaching program, recognizing the high importance of pollinators for the long-term sustainability of agriculture and food security.

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Agronomy SaskCanola Agronomy SaskCanola

Investigating interactions of ascospores and pycnidiospores with blackleg resistance in canola and efficacy of seed applied fungicides in these specific interactions in western Canada

This is the first investigation into the infection by pycnidio- and asco-spores, as well as a mixture via wounds under the influence of cultivar resistance and fungicide seed treatment for blackleg disease in canola. The findings from this study suggested that it is feasible to produce ascospores and pycnidiospores in the lab and use these types of spores to test canola materials for blackleg resistance in both controlled environment and field conditions.

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Agronomy SaskCanola Agronomy SaskCanola

New Clubroot Pathotypes and Second Generation Resistance

Clubroot poses a significant threat to canola growers. Although genetic resistance is critical for managing the disease, changes in pathogen virulence endanger its effectiveness. Second-generation resistance offers promise against pathotypes that can overcome first-generation resistance. However, without an understanding of pathogen virulence on second-generation resistance, we risk compromising its efficacy. This project provided data on the performance of second-generation clubroot-resistant cultivars in the field, along with insights into the virulence characteristics of pathogen populations collected from these cultivars.

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