Towards better understanding of Leptosphaeria-Brassica interactions via international collaborations to standardize the nomanclature of blackleg resistance genes

Term: 3 years, beginning in 2019
Status: Ongoing
Researcher(s): Hossein Borhan, AAFC
SaskCanola Investment: $105,000
Total Project Cost: $210,000
Funding Partners: Alberta Canola Producers Commission

Project Description

Our understanding of genetics and genomics of Leptosphaeria maculans-Brassica pathosystem has advanced rapidly in the past decade through the efforts of the international research community. However, due to the use of diverse Brassica and L. maculans genetic materials and markers, there is confusion over some of the resistance gene naming from Brassica species and their corresponding avirulence genes in L. maculans. Proper management of blackleg disease requires a clear understanding of the existing genetic capacity and pathogen virulence. In order to achieve this, we propose to coordinate the efforts of the international blackleg research community and generate a common set of plant and pathogen genetic resources and markers to be used as an international standard.

Objectives

  1. To expand the current B. napus host differential lines

  2. To generate a common set of L. maculans differential isolates

  3. To genotype the R gene(s) of current B. napus collections labeled as resistant to blackleg disease

  4. To create a common B. napus-Leptosphaeria database of genetics and genomics data as well as protocols

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From field to the genome. Application of third generation sequencing to direct genotyping of canola pathogens