Modified lipid metabolism to deliver improved low temperature tolerance in Brassica napus

Term: 4 years, beginning in 2019
Status: Ongoing
Researcher(s): Mark Smith, AAFC
SaskCanola Investment: $73,810
Total Project Cost: $268,400
Funding Partners: Agriculture Development Fund

Project Description

The output of the project will be a better understanding of the role of lipid composition in low temperature performance in B. napus seedlings. The objective is to identify new targets for breeding canola with improved low temperature characteristics. The work is long term, but has the advantage that much is known about the enzymes and corresponding genes involved in lipid metabolism in plants, information that will aid in the faster identification of useful markers and beneficial or negative alleles for introduction or removal in breeding programs.

Objectives

  1. Determine the membrane lipid composition of Brassica napus seeds and seedlings in lines with different seed oil composition.

  2. Test the hypothesis of a link between B. napus seed lipid composition and germination/seedling establishment at low temperature.

  3. Identify strategies to modify lipid metabolism to deliver improved, early season, low temperature tolerance in Brassica napus.

  4. Evolution of enhanced cold tolerance through iterative selection.

  5. The goal is to apply a targeted approach to identify new traits to confer improved low temperature tolerance in seedling canola.

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Increasing abiotic (drought) and biotic (clubroot) resistance in Brassica species (Arabidopsis and Canola) by modifying auxin response

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Determine the contribution of specific defence genes to Sclerotinia sclerotiorum resistance in canola (Brassica napus)