Defining Populations Of The L. maculans Pathogen In Test Sites Used For Canola Blackleg Resistance Trials

Researcher: Hossein Borhan, Agriculture and Agri-Food Canada, Saskatoon

Project Code: 3.3.3

Final Report: April 2013

Summary: Blackleg caused by Leptosphaeria maculans (LM) is a major disease of canola (Brassica napus) in Canada and world-wide. A solid understanding of the pathogenic variability of L. maculans populations is vital to maintaining effective resistance in future canola varieties. Researchers at Agriculture and Agri-Food Canada (AAFC) in Saskatoon led a three-year study to produce a set of single blackleg-resistance gene B.napus lines in a common susceptible background for the accurate pathotyping of L.maculans isolates. From the study seven B. napus resistant (BC5S3) lines were produced, and the final lines are expected to be 99.8% homozygous. This will prove to be extremely valuable to the blackleg research community both in Canada and worldwide. As well, results from the 2013 WCC Trials indicate that LepR1 and LepR2, which were identified and distributed to breeders by AAFC Saskatoon, appear to offer the most consistent level of protection to blackleg disease across Canadian locations.

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